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- Title
Spatiotemporal transcriptomic atlas reveals the dynamic characteristics and key regulators of planarian regeneration.
- Authors
Cui, Guanshen; Dong, Kangning; Zhou, Jia-Yi; Li, Shang; Wu, Ying; Han, Qinghua; Yao, Bofei; Shen, Qunlun; Zhao, Yong-Liang; Yang, Ying; Cai, Jun; Zhang, Shihua; Yang, Yun-Gui
- Abstract
Whole-body regeneration of planarians is a natural wonder but how it occurs remains elusive. It requires coordinated responses from each cell in the remaining tissue with spatial awareness to regenerate new cells and missing body parts. While previous studies identified new genes essential to regeneration, a more efficient screening approach that can identify regeneration-associated genes in the spatial context is needed. Here, we present a comprehensive three-dimensional spatiotemporal transcriptomic landscape of planarian regeneration. We describe a pluripotent neoblast subtype, and show that depletion of its marker gene makes planarians more susceptible to sub-lethal radiation. Furthermore, we identified spatial gene expression modules essential for tissue development. Functional analysis of hub genes in spatial modules, such as plk1, shows their important roles in regeneration. Our three-dimensional transcriptomic atlas provides a powerful tool for deciphering regeneration and identifying homeostasis-related genes, and provides a publicly available online spatiotemporal analysis resource for planarian regeneration research. Cui et al. present a comprehensive three-dimensional spatiotemporal transcriptome landscape of planarian regeneration. They identified a novel pluripotent neoblast subtype and new spatially specific genes essential to tissue regeneration.
- Subjects
REGENERATION (Biology); GENE regulatory networks; TRANSCRIPTOMES; GENE expression; FUNCTIONAL analysis
- Publication
Nature Communications, 2023, Vol 14, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-023-39016-0