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- Title
Fermented Rice Bran Supplementation Prevents the Development of Intestinal Fibrosis Due to DSS-Induced Inflammation in Mice.
- Authors
Agista, Afifah Zahra; Rusbana, Tubagus Bahtiar; Islam, Jahidul; Ohsaki, Yusuke; Sultana, Halima; Hirakawa, Ryota; Watanabe, Kouichi; Nochi, Tomonori; Ardiansyah; Budijanto, Slamet; Yang, Suh-Ching; Koseki, Takuya; Aso, Hisashi; Komai, Michio; Shirakawa, Hitoshi
- Abstract
Fermented rice bran (FRB) is known to protect mice intestines against dextran sodium sulfate (DSS)-induced inflammation; however, the restoration of post-colitis intestinal homeostasis using FRB supplementation is currently undocumented. In this study, we observed the effects of dietary FRB supplementation on intestinal restoration and the development of fibrosis after DSS-induced colitis. DSS (1.5%) was introduced in the drinking water of mice for 5 days. Eight mice were sacrificed immediately after the DSS treatment ended. The remaining mice were divided into three groups, comprising the following diets: control, 10% rice bran (RB), and 10% FRB-supplemented. Diet treatment was continued for 2 weeks, after which half the population of mice from each group was sacrificed. The experiment was continued for another 3 weeks before the remaining mice were sacrificed. FRB supplementation could reduce the general observation of colitis and production of intestinal pro-inflammatory cytokines. FRB also increased intestinal mRNA levels of anti-inflammatory cytokine, tight junction, and anti-microbial proteins. Furthermore, FRB supplementation suppressed markers of intestinal fibrosis. This effect might have been achieved via the canonical Smad2/3 activation and the non-canonical pathway of Tgf-β activity. These results suggest that FRB may be an alternative therapeutic agent against inflammation-induced intestinal fibrosis.
- Subjects
RICE; HOMEOSTASIS; INFLAMMATION; ANIMAL experimentation; FIBROSIS; WATER supply; MESSENGER RNA; COLITIS; INTESTINES; DEXTRAN; MICE
- Publication
Nutrients, 2021, Vol 13, Issue 6, p1869
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu13061869