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- Title
Molecular cloning of cDNAs for 14-3-3 and its protein interactions in a white-rot fungus Phanerochaete chrysosporium.
- Authors
Hu, Guoku; Feng, Hong; Zhang, Tao; Yan, Yonghong; Wu, Bo; Jiang, Quan; Wu, Jinming; Zhang, Yizheng
- Abstract
The 14-3-3 is a highly conserved, ubiquitous expressed protein in eukaryotes and very important in the regulation of such crucial cellular processes as metabolism, signal transduction, cell-cycle control, apoptosis, protein trafficking, transcription, stress responses, and malignant transformation. In this study, the full-length cDNA of 14-3-3 was cloned first time from a white-rot fungus Phanerochaete chrysosporium. Yeast two hybrid has been performed to fish out the proteins that can bind with 14-3-3 in P. chrysosporium. The results showed that 14-3-3 could form homodimers in P. chrysosporium. Two novel proteins containing predicted WD domain could interact with 14-3-3, too. In addition, the transcription of 14-3-3 gene under low-nitrogen medium was constitutional by RT-PCR analysis. These results indicate that 14-3-3 protein in P. chrysosporium may be involved in multiple cellular processes.
- Publication
Annals of Microbiology, 2006, Vol 56, Issue 3, p191
- ISSN
1590-4261
- Publication type
Article
- DOI
10.1007/BF03175004