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- Title
Autologous Platelet Rich Plasma Have Positive Effect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season.
- Authors
ALCAY, Selim; AKTAR, Ahmet; KOCA, Davut; KILIC, Mehmet Ali; AKKASOGLU, Mustafa; YILMAZ, Mehmet Melih; SAGIRKAYA, Hakan
- Abstract
The aim of this study was to evaluate the eff ects of autologous platelet-rich plasma (PRP) supplemented egg yolk based extender on ram semen aft er thawing. Semen samples were collected from nine Kıvırcık rams and each semen was divided into four equal aliquots and mixed with diff erent concentrations of PRP supported extenders [no PRP (control), 12.5x106/mL PRP, 25x106/mL PRP, or 50x106/mL PRP)]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed aft er thawing. Th e results showed that 25x106/mL PRP group had positive eff ect on motility (61.67±3.81), membrane functional integrity (71.00±2.96) and MDA levels (5.13±0.64) at post-thawed (P<0.05). It was determined that 25x106/mL PRP and 50x106/mL PRP groups were more eff ective than other groups in terms of mitochondrial membrane potential (69.50±1.93), acrosome integrity (78.04±2.65) and DNA integrity (5.33±0.92). Th e results of the study showed that autologous PRP has a protective eff ect in the cryopreservation of ram spermatozoa.
- Subjects
FROZEN semen; PLATELET-rich plasma; SPERMATOZOA; MEMBRANE potential; RAMS; MITOCHONDRIAL membranes; EGG yolk
- Publication
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2022, Vol 28, Issue 2, p229
- ISSN
1300-6045
- Publication type
Article
- DOI
10.9775/kvfd.2021.26763