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- Title
The Role of Probiotics in Inhibition Mechanism of Methicillin-Resistant Staphylococcus Aureus.
- Authors
Abbasi, Mahsa; Dolatabadi, Samaneh; Ghorbannezhad, Ghazaleh; Sharifi, Fatemeh; Rahimi, Hamid Reza
- Abstract
Down-regulation of the mecA gene is considered as a promising approach to control over antibiotic resistance in methicillin resistant Staphylococcus aureus (MRSA). This in vitro study evaluated the ability of synbiotics to inhibit the growth of MRSA and modify the expression of mecA. Moreover, susceptibility testing was performed to investigate synbioticinduced changes in MRSA resistance. MRSA isolates were collected from different clinical specimens and explored for antibiotic susceptibility using both disk-agar diffusion method as well as polymerase chain reaction (PCR) for detection of mecA gene. Synbiotics in the form of Kidilacto, Vitalacto, and Protexino sachets were applied to prepare cell-free culture supernatants. Their antibacterial activity was determined by disk and well diffusion methods. The impact of synbiotics on the expression of mecA that denotes penicillin susceptibility was tested by real time PCR. It was shown that the synbiotics produced components with antimicrobial activities against MRSA. The supernatant produced by synbiotics can afford to confer penicillin susceptibility in the MRSA isolates in a timedependent fashion. Two third of susceptible MRSA isolates carried decreased levels of mecA expression. In conclusion, synbiotics are effective for reducing MRSA growth and antibiotic resistance through suppression of mecA.
- Subjects
PROBIOTICS; METHICILLIN-resistant staphylococcus aureus; PENICILLIN; POLYMERASE chain reaction; DRUG resistance in bacteria
- Publication
McGill Journal of Medicine, 2020, Vol 18, Issue 1, p82
- ISSN
1201-026X
- Publication type
Article
- DOI
10.26443/mjm.v18i1.156