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- Title
Generating pluripotent stem cells: Differential epigenetic changes during cellular reprogramming
- Authors
Tobin, Stacey C.; Kim, Kitai
- Abstract
Abstract: Pluripotent stem cells hold enomous potential for therapuetic applications in tissue replacement therapy. Reprogramming somatic cells from a patient donor to generate pluripotent stem cells involves both ethical concerns inherent in the use of embryonic and oocyte-derived stem cells, as well as issues of histocompatibility. Among the various pluripotent stem cells, induced pluripotent stem cells (iPSC)—derived by ectopic expression of four reprogramming factors in donor somatic cells—are superior in terms of ethical use, histocompatibility, and derivation method. However, iPSC also show genetic and epigenetic differences that limit their differentiation potential, functionality, safety, and potential clinical utility. Here, we discuss the unique characteristics of iPSC and approaches that are being taken to overcome these limitations.
- Subjects
PLURIPOTENT stem cells; EPIGENETICS; CELL differentiation; TRANSPLANTATION of organs, tissues, etc.; HISTOCOMPATIBILITY; SOMATIC cells; GENE expression
- Publication
FEBS Letters, 2012, Vol 586, Issue 18, p2874
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.07.024