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- Title
Efficient T-Cell Compartment in HIV-Positive Patients Receiving Orthotopic Liver Transplant and Immunosuppressive Therapy.
- Authors
Franceschini, Erica; Biasi, Sara De; Digaetano, Margherita; Bianchini, Elena; Tartaro, Domenico Lo; Gibellini, Lara; Menozzi, Marianna; Zona, Stefano; Tarantino, Giuseppe; Nasi, Milena; Codeluppi, Mauro; Guaraldi, Giovanni; Magistri, Paolo; Benedetto, Fabrizio Di; Pinti, Marcello; Mussini, Cristina; Cossarizza, Andrea; De Biasi, Sara; Lo Tartaro, Domenico; Di Benedetto, Fabrizio
- Abstract
<bold>Background: </bold>In patients undergoing orthotopic liver transplant (OLT), immunosuppressive treatment is mandatory and infections are leading causes of morbidity/mortality. Thus, it is essential to understand the functionality of cell-mediated immunity after OLT. The aim of the study was to identify changes in T-cell phenotype and polyfunctionality in human immunodeficiency virus-positive (HIV+) and -negative (HIV-) patients undergoing immunosuppressive treatment after OLT.<bold>Methods: </bold>We studied peripheral blood mononuclear cells from 108 subjects divided into 4 groups of 27: HIV+ transplanted patients, HIV- transplanted patients, HIV+ nontransplanted patients, and healthy subjects. T-cell activation, differentiation, and cytokine production were analyzed by flow cytometry.<bold>Results: </bold>Median age was 55 years (interquartile range, 52-59 years); the median CD4 count in HIV+ patients was 567 cells/mL, and all had undetectable viral load. CD4+ and CD8+ T-cell subpopulations showed different distributions between HIV+ and HIV- OLT patients. A cluster representing effector cells expressing PD1 was abundant in HIV- transplanted patients and they were characterized by higher levels of CD4+ T cells able to produce interferon-γ and tumor necrosis factor-α.<bold>Conclusions: </bold>HIV- transplanted patients have more exhausted or inflammatory T cells compared to HIV+ transplanted patients, suggesting that patients who have already experienced a form of immunosuppression due to HIV infection respond differently to anti-rejection therapy.
- Subjects
LIVER transplantation; IMMUNOSUPPRESSIVE agents; HIV-positive persons; HIV infections; CELLULAR immunity; BK virus; CYTOKINES; RESEARCH; MONONUCLEAR leukocytes; CROSS-sectional method; VIRAL load; RESEARCH methodology; IMMUNOSUPPRESSION; CASE-control method; MEDICAL cooperation; EVALUATION research; INTERFERONS; COMPARATIVE studies; CD4 lymphocyte count; T cells; HIV
- Publication
Journal of Infectious Diseases, 2021, Vol 223, Issue 3, p482
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiaa395