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- Title
Prostaglandin H<sub>2</sub>-derived adducts of proteins correlate with Alzheimer's disease severity.
- Authors
Zagol-Ikapitte, Irene; Masterson, Tina S.; Amarnath, V.; Montine, Thomas J.; Andreasson, Katrin I.; Boutaud, Olivier; Oates, John A.
- Abstract
The formation of cyclooxygenase-derived lipid adducts of protein in brains of patients who had Alzheimer's disease has been investigated. The enzymatic product of the cyclooxygenases, prostaglandin H2, rearranges in part to highly reactive γ-ketoaldehydes, levuglandin (LG) E2 and LGD2. These γ-ketoaldehydes react with free amines on proteins to yield a covalent adduct. Utilizing analysis of the levuglandinyl-lysine adducts by liquid chromatography-tandem mass spectrometry, we now find that this post-translational modification is increased significantly in the hippocampus in Alzheimer's disease. The magnitude of the increase correlates with the pathological evidence of severity.
- Subjects
PROSTAGLANDINS; CYCLOOXYGENASES; PROTEINS; BRAIN chemistry; ALZHEIMER'S disease; AMINES
- Publication
Journal of Neurochemistry, 2005, Vol 94, Issue 4, p1140
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2005.03264.x