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- Title
VIABILITY AND MOLECULAR AUTHENTICATION OF Coccidioides spp. ISOLATES FROM THE INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO CULTURE COLLECTION, BRAZIL.
- Authors
CAVALCANTI, Sarah Desiree Barbosa; VIDAL, Monica Scarpelli Martinelli; SOUSA, Maria da Glòria Teixeira de; DEL NEGRO, Gilda Maria Barbaro
- Abstract
Coccidioidomycosis is an emerging fungal disease in Brazil; adequate maintenance and authentication of Coccidioides isolates are essential for research into genetic diversity of the environmental organisms, as well as for understanding the human disease. Seventeen Coccidioides isolates maintained under mineral oil since 1975 in the Instituto de Medicina Tropical de Sao Paulo (IMTSP) culture collection, Brazil, were evaluated with respect to their viability, morphological characteristics and genetic features in order to authenticate these fungal cultures. Only five isolates were viable after almost 30 years, showing typical morphological characteristics, and sequencing analysis using Coi-F and Coi-R primers revealed 99% identity with Coccidioides genera. These five isolates were then preserved in liquid nitrogen and sterile water, and remained viable after two years of storage under these conditions, maintaining the same features.
- Subjects
BRAZIL; MYCOSES; COCCIDIOIDOMYCOSIS; DNA primers; LIQUID nitrogen; FUNGAL cultures; NUCLEOTIDE sequence; TROPICAL medicine
- Publication
Revista do Instituto de Medicina Tropical de São Paulo, 2013, Vol 55, Issue 1, p7
- ISSN
0036-4665
- Publication type
Article
- DOI
10.1590/S0036-46652013000100002