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- Title
Dengue-specific subviral nanoparticles: design, creation and characterization.
- Authors
Khetarpal, Niyati; Poddar, Ankur; Nemani, Satish K.; Dhar, Nisha; Patil, Aravind; Negi, Priyanka; Perween, Ashiya; Viswanathan, Ramaswamy; Lünsdorf, Heinrich; Tyagi, Poornima; Raut, Rajendra; Arora, Upasana; Jain, Swatantra K.; Rinas, Ursula; Swaminathan, Sathyamangalam; Khanna, Navin
- Abstract
Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate. Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally. Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence. Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.
- Subjects
NANOCAPSULES; DENGUE; VIRUS-like particles; DENGUE viruses; HEPATITIS B virus; CELL surface antigens
- Publication
Journal of Nanobiotechnology, 2013, Vol 11, Issue 1, p1
- ISSN
1477-3155
- Publication type
Article
- DOI
10.1186/1477-3155-11-15