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- Title
Novel Tripod Amphiphiles for Membrane Protein Analysis.
- Authors
Chae, Pil Seok; Kruse, Andrew C.; Gotfryd, Kamil; Rana, Rohini R.; Cho, Kyung Ho; Rasmussen, Søren G. F.; Bae, Hyoung Eun; Chandra, Richa; Gether, Ulrik; Guan, Lan; Kobilka, Brian K.; Loland, Claus J.; Byrne, Bernadette; Gellman, Samuel H.
- Abstract
Integral membrane proteins play central roles in controlling the flow of information and molecules across membranes. Our understanding of membrane protein structures and functions, however, is seriously limited, mainly due to difficulties in handling and analysing these proteins in aqueous solution. The use of a detergent or other amphipathic agents is required to overcome the intrinsic incompatibility between the large lipophilic surfaces displayed by the membrane proteins in their native forms and the polar solvent molecules. Here, we introduce new tripod amphiphiles displaying favourable behaviours toward several membrane protein systems, leading to an enhanced protein solubilisation and stabilisation compared to both conventional detergents and previously described tripod amphiphiles.
- Subjects
AMPHIPHILES; MEMBRANE proteins; AQUEOUS solutions; HYDROPHOBIC surfaces; PROTEIN solubility; POLAR solvents; DETERGENTS
- Publication
Chemistry - A European Journal, 2013, Vol 19, Issue 46, p15645
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201301423