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- Title
Cytological and molecular characterization of a novel monogenic dominant GMS in Brassica napus L.
- Authors
Ai-Guang Guo; Dian-Rong Li; Jian-Hua Tian; Fei Huang; Gen-Lou Sun
- Abstract
Abstract  A novel genic male sterile (GMS) line in Brassica napus L., which was identified in 1999, was found to be controlled by a monogenic dominant gene, which we have designated as MDGMS. The microspores of the MDGMS abort before the degradation of the tapetal cell layer. The F1 fertility from any fertile lines crossed with MDGMS segregated and the ratio was close to 1:1. Bulked segregation analysis (BSA) was employed to identify random amplified polymorphic DNA (RAPD) markers linked to the Ms gene in MDGMS. Among 880 random 10-mer oligonucleotide primers screened against the bulk DNA of sterile and fertile, one primer S243 (5â²-CTATGCCGAC-3â²) gave a repeatable 1500-bp DNA polymorphic segment S2431500 between the two bulks. Analysis of individual plants of each bulks and other types of GMS and cytoplasmic male sterility (CMS) lines suggest that the RAPD marker S2431500 is closely linked to the MDGMS locus in rapeseed. This RAPD marker has been converted into sequence characterized amplified region (SCAR) marker to aid identification of male-fertility genotypes in segregating progenies of MDGMS in marker-assisted selection (MAS) breeding programs.
- Subjects
BRASSICA; DNA; NUCLEIC acids; GENETIC polymorphisms
- Publication
Plant Cell Reports, 2007, Vol 26, Issue 5, p571
- ISSN
0721-7714
- Publication type
Article