We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Ultraviolet-B radiation (280–315 nm) invoked antioxidant defence systems in Vigna unguiculata (L.) Walp. and Crotalaria juncea L.
- Authors
V. Selvakumar
- Abstract
Abstract A crop legume Vigna unguiculata L. (Walp.) and a wild legume Crotalaria juncea L. were evaluated for their relative responses to the oxidative stress injury induced by various doses of UV-B radiation (UV-B, 280–315 nm; 0, 1.0, 1.4, 4.7, and 6.0 kJ m−2 d−1). A dose-dependent damage in lipid peroxidation was determined as an index of membrane injury caused by UV-B. The impact was significantly higher in V. unguiculata than in C. juncea. The specific activities of superoxide dismutase, ascorbate peroxidase, monodehydroascorbate reductase, and dehydroascorbate reductase increased directly proportional to UV-B doses. However, the activities of these enzymes were significantly higher in V. unguiculata than in C. juncea indicating that V. unguiculata was inflicted with more severe oxidative stress injury under UV-B. In C. juncea the glutathione reductase and ascorbate oxidase activities were 35 and 40 % greater than in V. unguiculata, respectively. Further, the non-enzymatic antioxidants ascorbate and glutathione, and their reduced/oxidizes ratios in C. juncea were much greater than V. unguiculata indicating C. juncea has an inherently greater antioxidative potential than V. unguiculata. Thus C. juncea is better adapted to oxidative stress than V. unguiculata by means of efficient cellular antioxidant mechanisms helping to combat the photooxidative stress injury elicited by UV-B.
- Subjects
OXIDATIVE stress; CROTALARIA; CROTALARIA spectabilis; OLIGOPEPTIDES
- Publication
Photosynthetica, 2008, Vol 46, Issue 1, p98
- ISSN
0300-3604
- Publication type
Article
- DOI
10.1007/s11099-008-0017-9