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- Title
Monitoring dynamic alterations in calcium homeostasis by T<sub>1</sub>-mapping manganese-enhanced MRI (MEMRI) in the early stage of small intestinal ischemia-reperfusion injury.
- Authors
Zhao, Da‐wei; Zhang, Le‐tian; Cheng, Hai‐yun; Zhang, Yu‐long; Min, Jia‐yan; Xiao, Hua‐liang; Wang, Yi
- Abstract
Manganese-enhanced MRI studies have proven to be useful in monitoring physiological activities associated with calcium ions (Ca2+) due to the paramagnetic property of the manganese ion (Mn2+), which makes it an excellent probe of Ca2+. In this study, we developed a method in which a Mn2+-enhanced T1-map MRI could enable the monitoring of Ca2+ influx during the early stages of intestinal ischemia-reperfusion (I/R) injury. The Mn2+ infusion protocol was optimized by obtaining dose-dependent and time-course wash-out curves using a Mn2+-enhanced T1-map MRI of rabbit abdomens following an intravenous infusion of 50 mmol/l MnCl2 (5-10 nmol/g body weight (BW)). In the rabbit model of intestinal I/R injury, T1 values were derived from the T1 maps in the intestinal wall region and revealed a relationship between the dose of the infused MnCl2 and the intestinal wall relaxation time. Significant Mn2+ clearance was also observed over time in control animals after the infusion of Mn2+ at a dose of 10 nmol/g BW. This technique was also shown to be sensitive enough to monitor variations in calcium ion homeostasis in vivo after small intestinal I/R injury. The T1 values of the intestinal I/R group were significantly lower ( P < 0.05) than that of the control group at 5, 10, and 15 min after Mn2+ infusion. Our data suggest that MnCl2 has the potential to be an MRI contrast agent that can be effectively used to monitor changes in intracellular Ca2+ homeostasis during the early stages of intestinal I/R injury. Copyright © 2015 John Wiley & Sons, Ltd.
- Publication
NMR in Biomedicine, 2015, Vol 28, Issue 8, p958
- ISSN
0952-3480
- Publication type
Article
- DOI
10.1002/nbm.3335