We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Experimental system to detect a labeled cell monolayer in a microfluidic environment.
- Authors
Gargam, Nicolas; Darrasse, Luc; Raynaud, Jean‐Sebastien; Ginefri, Jean‐Christoph; Robert, Philippe; Poirier‐Quinot, Marie
- Abstract
<bold>Purpose: </bold>To investigate the feasibility of detecting a living cell monolayer labeled with gadoterate (Gd-DOTA) in a microfluidic environment, by micromagnetic resonance imaging (MRI) in a 2.35T small-animal system. The development of new targeted contrast agents (CAs) requires proof-of-concept studies in order to establish the detectability of the CA and to predict the role of biodistribution in its uptake mechanisms. A promising approach is to carefully mimic the in vivo pharmacokinetic context with reduced experimental complexity compared to in vivo situations.<bold>Materials and Methods: </bold>A dedicated experimental system was built by combining a microfluidic slide and a radiofrequency probe based on a 6 mm diameter multiturn transmission-line resonator. Adherent KB cells were incubated with different concentrations of Gd. MRI data were acquired at 2.35T with a 3D gradient echo and a resolution of 12.4 μm perpendicular to the cell layer. The longitudinal relaxation rate, R1 , was measured as a function of the amount of Gd internalized by the cells.<bold>Results: </bold>R1 measurements for different Gd concentrations per cell were performed using data with an signal-to-noise ratio (SNR) of 100. Relaxation-rate variations ΔR1 of 0.035 s(-1) were measured. A quenching effect was observed at Gd concentrations above 20 fmol/cell.<bold>Conclusion: </bold>Our results suggest that this dedicated experimental system is suitable for specifically assessing new high-relaxivity targeted CAs under real-time uptake conditions.
- Subjects
MAGNETIC resonance imaging equipment; ANIMAL experimentation; CELL physiology; CELLS; HETEROCYCLIC compounds; MAGNETIC resonance imaging; ORGANOMETALLIC compounds; STAINS &; staining (Microscopy); TUMORS; BIOCHIPS; PILOT projects; PRODUCT design; CONTRAST media; MEDICAL equipment reliability
- Publication
Journal of Magnetic Resonance Imaging, 2015, Vol 42, Issue 4, p1100
- ISSN
1053-1807
- Publication type
journal article
- DOI
10.1002/jmri.24893