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- Title
Bioanalytical Method Development and Validation of Veratraldehyde and Its Metabolite Veratric Acid in Rat Plasma: An Application for a Pharmacokinetic Study.
- Authors
Huh, Hyun Wook; Song, Hee-Yong; Na, Young-Guk; Kim, Minki; Han, Mingu; Pham, Thi Mai Anh; Lee, Hyeonmin; Suh, Jungkyu; Lee, Seok-Jong; Lee, Hong-Ki; Cho, Cheong-Weon; Yoon, In-Soo; Cho, Hyun-Jong; Zacharis, Constantinos
- Abstract
A simple, sensitive, and rapid UHPLC-MS/MS method was developed for the simultaneous determination of veratraldehyde and its metabolite veratric acid in rat plasma. Cinnamaldehyde was used as an internal standard (IS) and the one-step protein precipitation method with 0.2% formic acid in acetonitrile (mobile phase B) was used for the sample extraction. Reversed C18 column (YMC-Triart C18 column, 50 mm × 2.0 mm, 1.9 µm) was used for chromatographic separation and was maintained at 30 °C. The total run time was 4.5 min and the electrospray ionization in positive mode was used with the transition m/z 167.07 → 139.00 for veratraldehyde, m/z 183.07 → 139.00 for veratric acid, and m/z 133.00 → 55.00 for IS. The developed method exhibited good linearity (r2 ≥ 0.9977), and the lower limits of quantification ranged from 3 to 10 ng/mL for the two analytes. Intra-day precision and accuracy parameters met the criteria (within ±15%) during the validation. The bioanalytical method was applied for the determination of veratraldehyde and veratric acid in rat plasma after oral and percutaneous administration of 300 and 600 mg/kg veratraldehyde. Using the analytical methods established in this study, we can confirm the absorption and metabolism of veratraldehyde in rats for various routes.
- Subjects
PHARMACOKINETICS; RATS; ACIDS
- Publication
Molecules, 2020, Vol 25, Issue 12, p2800
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules25122800