We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Screening 89 Pesticides in Fishery Drugs by Ultrahigh Performance Liquid Chromatography Tandem Quadrupole-Orbitrap Mass Spectrometer.
- Authors
Wang, Shou-Ying; Kong, Cong; Chen, Qing-Ping; Yu, Hui-Juan; Locatelli, Marcello; Tartaglia, Angela; Melucci, Dora; Kabir, Abuzar; Ulusoy, Halil Ibrahim; Samanidou, Victoria; Gikas, Evagelos; Chaimbault, Patrick
- Abstract
Multiclass screening of drugs with high resolution mass spectrometry is of great interest due to its high time-efficiency and excellent accuracy. A high-scale, fast screening method for pesticides in fishery drugs was established based on ultrahigh performance liquid chromatography tandem quadrupole-Orbitrap high-resolution mass spectrometer. The target compounds - were diluted in methanol and extracted by ultrasonic treatment, and the extracts were diluted with MeOH-water (1:1, v/v) and centrifuged to remove impurities. The chromatographic separation was performed on an Accucore aQ-MS column (100 mm × 2.1 mm, 2.6 μm) with gradient elution using 0.1% formic acid in water (containing 5 mmol/L ammonium formate) and 0.1% formic acid in methanol (containing 5 mmol/L ammonium formate) in Full Scan/dd-MS2 (TopN) scan mode. A screening database, including mass spectrometric and chromatographic information, was established for identification of compounds. The screening detection limits of methods ranged between 1–500 mg/kg, the recoveries of real samples spiked with the concentration of 10 mg/kg and 100 mg/kg standard mixture ranged from 70% to 110% for more than sixty compounds, and the relative standard deviations (RSDs) were less than 20%. The application of this method showed that target pesticides were screened out in 10 samples out of 21 practical samples, in which the banned pesticide chlorpyrifos were detected in 3 out of the 10 samples.
- Subjects
MASS spectrometers; LIQUID chromatography; PESTICIDES; SUPERCRITICAL fluid chromatography; MASS spectrometry; FISHERIES; FENITROTHION; ORGANOPHOSPHORUS pesticides
- Publication
Molecules, 2019, Vol 24, Issue 18, p3375
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules24183375