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- Title
Ultra-Small Pd(0) Nanoparticles into a Designed Semisynthetic Lipase: An Efficient and Recyclable Heterogeneous Biohybrid Catalyst for the Heck Reaction under Mild Conditions.
- Authors
Lopez-Tejedor, David; de las Rivas, Blanca; Palomo, Jose M.
- Abstract
A novel heterogeneous enzyme-palladium (Pd) (0) nanoparticles (PdNPs) bionanohybrid has been synthesized by an efficient, green, and straightforward methodology. A designed Geobacillus thermocatenulatus lipase (GTL) variant genetically and then chemically modified by the introduction of a tailor-made cysteine-containing complementary peptide- was used as the stabilizing and reducing agent for the in situ formation of ultra-small PdNPs nanoparticles embedded on the protein structure. This bionanohybrid was an excellent catalyst in the synthesis of trans-ethyl cinnamate by Heck reaction at 65 °C. It showed the best catalytic performance in dimethylformamide (DMF) containing 10-25% of water as a solvent but was also able to catalyze the reaction in pure DMF or with a higher amount of water as co-solvent. The recyclability and stability were excellent, maintaining more than 90% of catalytic activity after five cycles of use.
- Subjects
CYSTEINE; DIMETHYLFORMAMIDE; GEOBACILLUS stearothermophilus; CINNAMATES; PROTEIN structure
- Publication
Molecules, 2018, Vol 23, Issue 9, p2358
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules23092358