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- Title
Live cell imaging fails to support viral-protein-mediated intercellular trafficking.
- Authors
Wu, Fuqing; Long, Jing; Wang, Shuai; Xing, Junji; Li, Meili; Zheng, Chunfu
- Abstract
The herpes simplex virus type I protein VP22 has been reported to have the property of intercellular trafficking. However, there is little direct evidence to demonstrate that VP22 can shuttle freely between living cells. Here, we employ a novel and simple assay using live cell fluorescence microscopy to investigate the intercellular transport property. Our results demonstrated that VP22, bovine herpesvirus-1 VP22, HSV-1 US11 and HIV Tat could not shuttle into neighboring cells via direct contact.
- Subjects
FLUORESCENCE microscopy; BOVINE herpesvirus-1; HERPES simplex virus; HOST-virus relationships; MICROBIOLOGICAL assay; VIRUS diseases in cattle; VIRAL proteins
- Publication
Archives of Virology, 2012, Vol 157, Issue 7, p1383
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-012-1308-9