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- Title
Development of Novel Polymorphic Microsatellite Markers and Their Application for Closely Related Camellia (Theaceae) Species.
- Authors
Yin, X.; Li, T.; Tian, Q. Q.; Dong, L.; Xu, L. A.; Wen, Q.
- Abstract
Camellia L. is a large genus with a total of 280 species, most of which have high ornamental value and oil production. Due to the abundant morphological variation, overlapping distribution, and even natural hybridization in the natural environment, many closely related species have similar phenotypes and complex relationships. Chloroplast microsatellite is an important measure for studies on phylogeny, genetic diversity, parental analysis, and molecular identification. In this study, a batch of polymorphic cpSSRs was developed by comparing the sequence variation of the same cpSSR locus based on the complete chloroplast genomes of nine Camellia species. Different Camellia species and individuals were used to test the interspecific versatility and polymorphism of cpSSRs, and 20 polymorphic cpSSR markers with high transferability in Camellia were detected. 10 out of 20 cpSSRs were selected for the studies on genetic diversity and molecular identification of five Camellia relatives by capillary electrophoresis. The results showed that C. chekiangoleosa had the highest genetic diversity among five species, with h and I being 0.434 and 0.714, respectively. We found that there was a close relationship (D = 0.1917) between C. chekiangoleosa and C. lucidissima, which could provide a molecular evidence for the merger of them. In addition, the haplotype diversity revealed that DNA barcodes constructed by combining the cpSSRs which had unique haplotypes could effectively contribute to the molecular identification of C. chekiangoleosa and its relatives.
- Subjects
MICROSATELLITE repeats; CAMELLIAS; CHLOROPLAST DNA; SPECIES; CAPILLARY electrophoresis; GENETIC variation
- Publication
Russian Journal of Genetics, 2022, Vol 58, Issue 4, p404
- ISSN
1022-7954
- Publication type
Article
- DOI
10.1134/S1022795422040147