We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Effect of CTX prophage deletion in cholera agent on expression of regulatory genes controlling virulence and biofilm formation.
- Authors
Smirnova, N.; Agafonov, D.; Kul'shan', T.; Shchelkanova, E.; Krasnov, Ya.; Lozovsky, Yu.; Kutyrev, V.
- Abstract
This work represents the results of comparative analysis of the nucleotide sequences in the chromosomal region containing CTXφ prophage in isogenic toxigenic (Tox) and nontoxigenic (Tox) strains of Vibrio cholerae biovar El Tor. It is shown that spontaneous mutants which have identical Tox phenotype are formed either owing to the precise elimination of CTX prophage from the chromosome (deletion size is 6.9 kb) or owing to imprecise elimination with the additional loss of two other prophages (RS1φ and TLC), which are adjacent to CTX prophage (deletion size is 17.4 kb). It is revealed that, in the nontoxigenic mutants, CTX deletion initiates simultaneous change in several phenotypic properties, which are associated with virulence or biofilm formation, such as colonizing ability, HA/P production, VPS, and motility. For the first time, it is stated that the cause of the pleiotropic effect of the CTX deletion is a cascade decrease in the transcription levels of seven regulatory genes ( toxR, aphA, tcpP, tcpH, toxT, vpsT, vpsR) which control virulence and biofilm formation processes in the cholera agent.
- Subjects
CHOLERA toxin; REGULATOR genes; GENETIC regulation; NUCLEOTIDE sequence; DELETION mutation; GENETICS
- Publication
Russian Journal of Genetics, 2017, Vol 53, Issue 3, p302
- ISSN
1022-7954
- Publication type
Article
- DOI
10.1134/S1022795417020119