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- Title
Interleukin-20 induced cell death in renal epithelial cells and was associated with acute renal failure.
- Authors
Li, H.-H.; Hsu, Y.-H.; Wei, C.-C.; Lee, P.-T.; Chen, W.-C.; Chang, M.-S.
- Abstract
Acute renal failure is an abrupt decrease in renal function. Interleukin (IL)-10 inhibits ischemic and cisplatin-induced acute renal failure. We aimed to determine whether IL-20 affects renal tubular epithelial cells and is associated with acute renal failure. We analyzed the expression of IL-20 and its receptor (R) in the kidneys of rats with HgCl2-induced acute renal failure. Reverse transcription-PCR showed upregulated IL-20, and its receptors and immunohistochemical staining showed strongly expressed IL-20 protein in proximal tubular epithelial cells. We analyzed human proximal tubular epithelial (HK-2) cells, which expressed both IL-20 and its receptors. IL-20 specifically induced mitochondria-dependent apoptosis by activating caspase 9 in HK-2 cells. IL-20 also activated c-Jun N-terminal kinase and extracellular signal-regulated kinase 1/2, the downstream signals implicated in the apoptosis of HK-2 cells. Furthermore, IL-20 upregulated the transcripts of transforming growth factor (TGF)-β1, a critical mediator of renal injury. In hypoxic HK-2 cells, IL-20 and IL-22R1 transcripts increased, and IL-20 upregulated IL-1β transcripts. In vivo study further demonstrated that anti-IL-20 antibody reduced the expression of TGF-β1 and IL-1β and the number of damaged tubular cells in the kidneys of rats with acute renal failure. We concluded that IL-20 may be involved in the injury of renal epithelial cells in acute renal failure.Genes and Immunity (2008) 9, 395–404; doi:10.1038/gene.2008.28; published online 22 May 2008
- Subjects
ACUTE kidney failure; INTERLEUKINS; CELL death; EPITHELIAL cells; LABORATORY rats
- Publication
Genes & Immunity, 2008, Vol 9, Issue 5, p395
- ISSN
1466-4879
- Publication type
Article
- DOI
10.1038/gene.2008.28