We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Acid secretion of parietal cells is paralleled by a redistribution of NSF and α, β-SNAPs and inhibited by tetanus toxin.
- Authors
Lehnardt, Seija; Ahnert-Hilger, Gudrun; Bigalke, Hans; Jöns, Thomas
- Abstract
Stimulation of parietal cells causes fusion of intracellular tubulovesicles with the canalicular plasma membrane thereby increasing the apical membrane area up to tenfold. The presence of the SNARE proteins synaptobrevin, syntaxin1, and SNAP25 in parietal cells and their intracellular redistribution after stimulation suggest a SNARE-mediated mechanism. Here we show that NSF and α, β-SNAPs which are involved in the dissociation of the SNARE complex in neurons also occur in parietal cells exhibiting subcellular distributions similar to the ones obtained for SNARE proteins and for the H+, K+-ATPase. More importantly proteolytic cleavage of synaptobrevin by tetanus neurotoxin completely inhibits the cAMP-dependent increase of acid secretion further supporting the crucial role SNARE proteins play in parietal cells.
- Subjects
TETANUS toxin; TETANUS antitoxin; CLOSTRIDIUM diseases; BIOLOGICAL transport; NERVOUS system; NEUROTOXIC agents; BIOMOLECULES
- Publication
Histochemistry & Cell Biology, 2000, Vol 114, Issue 5, p387
- ISSN
0948-6143
- Publication type
Article
- DOI
10.1007/s004180000206