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- Title
Apaf-1 and caspase-9 do not act as tumor suppressors in myc-induced lymphomagenesis or mouse embryo fibroblast transformation.
- Authors
Scott, Clare L.; Schuler, Martin; Marsden, Vanessa S.; Egle, Alex; Pellegrini, Marc; Nesic, Dobrila; Gerondakis, Steve; Nutt, Stephen L.; Green, Douglas R.; Strasser, Andreas
- Abstract
Based on experiments with cultured fibroblasts, the apoptosis regulators caspase-9 and Apaf-1 are hypothesized to function as tumor suppressors. To investigate their in vivo role in lymphomagenesis, an IgH enhancer-driven c-myc transgene was crossed onto Apaf-1[sup-/-] and caspase-9[sup-/-] mice. Due to perinatal lethality, Eμ-myc transgenic Apaf-1[sup-/-] or caspase-9[sup-/-] fetal liver cells were used to reconstitute lethally irradiated recipient mice. Surprisingly, no differences were seen in rate, incidence, or severity of lymphoma with loss of Apaf-1 or caspase-9, and Apaf-1 was not a critical determinant of anticancer drug sensitivity of c-myc-induced tymphomas. Moreover, loss of Apaf-1 did not promote oncogene-induced transformation of mouse embryo fibroblasts. Thus, Apaf-1 and caspase-9 do not suppress c-myc-induced lymphomagenesis and embryo fibroblast transformation.
- Subjects
FIBROBLASTS; CELL death; ENZYME regulation; TUMOR suppressor proteins; LIVER cells; LYMPHOMAS; ANTINEOPLASTIC agents
- Publication
Journal of Cell Biology, 2004, Vol 164, Issue 1, p89
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200310041