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- Title
Isolation and characteristics of intrinsic myosin in mantle pallial cell layer of scallop.
- Authors
Araki, Takahiro; Hasegawa, Yasushi
- Abstract
SUMMARY: It has been reported that catch muscle type myosin is expressed in various tissues of scallop, including gonad, heart, foot and mantle. However, there has been no report about the isolation and identification of myosin from tissues other than the adductor muscle. We isolated myosin from the mantle pallial cell layer of scallop and compared the structure and the characteristics with those of striated and catch muscle myosins. Comparison among three kinds of myosins in cleavage patterns by trypsin and cyanogen bromide revealed that myosin in mantle pallial cell layer (pallial cell myosin) has a different structure from striated and catch muscle myosins. The Mg2+-ATPase activity of pallial cell myosin is significantly lower compared with those of striated and catch muscle myosins. The present results indicate the existence of the intrinsic myosin in mantle pallial cell layer.
- Subjects
MYOSIN; SCALLOPS; ADENOSINE triphosphatase; PHYSIOLOGY
- Publication
Fisheries Science, 2000, Vol 66, Issue 4, p743
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1046/j.1444-2906.2000.00121.x