We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Two Silene vulgaris copper transporters residing in different cellular compartments confer copper hypertolerance by distinct mechanisms when expressed in Arabidopsis thaliana.
- Authors
Li, Yanbang; Iqbal, Mazhar; Zhang, Qianqian; Spelt, Cornelis; Bliek, Mattijs; Hakvoort, Henk W. J.; Quattrocchio, Francesca M.; Koes, Ronald; Schat, Henk
- Abstract
Silene vulgaris is a metallophyte of calamine, cupriferous and serpentine soils all over Europe. Its metallicolous populations are hypertolerant to zinc (Zn), cadmium (Cd), copper (Cu) or nickel (Ni), compared with conspecific nonmetallicolous populations. These hypertolerances are metal-specific, but the underlying mechanisms are poorly understood., We investigated the role of HMA5 copper transporters in Cu-hypertolerance of a S. vulgaris copper mine population., Cu-hypertolerance in Silene is correlated and genetically linked with enhanced expression of two HMA5 paralogs, Sv HMA5I and Sv HMA5 II, each of which increases Cu tolerance when expressed in Arabidopsis thaliana. Most Spermatophytes, except Brassicaceae, possess homologs of Sv HMA5I and Sv HMA5 II, which originate from an ancient duplication predating the appearance of spermatophytes. Sv HMA5 II and the A. thaliana homolog At HMA5 localize in the endoplasmic reticulum and upon Cu exposure move to the plasma membrane, from where they are internalized and degraded in the vacuole. This resembles trafficking of mammalian homologs and is apparently an extremely ancient mechanism. Sv HMA5I, instead, neofunctionalized and always resides on the tonoplast, likely sequestering Cu in the vacuole., Adaption of Silene to a Cu-polluted soil is at least in part due to upregulation of two distinct HMA5 transporters, which contribute to Cu hypertolerance by distinct mechanisms.
- Subjects
SILENE vulgaris; PLANT genetic transformation; ZINC oxide; ARABIDOPSIS thaliana; HOMOLOGY (Biology); ENDOPLASMIC reticulum; PLANTS
- Publication
New Phytologist, 2017, Vol 215, Issue 3, p1102
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.14647