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- Title
Silencing of astrocyte elevated gene-1 inhibits proliferation and migration of melanoma cells and induces apoptosis.
- Authors
Zhang, Yue; Peng, Ge; Wang, Ying; Cui, Lixia; Wu, Wenqing; Wang, Luan; Liu, Chengyu; Han, Xiuping
- Abstract
Melanoma is an aggressive skin malignancy with a high mortality. Astrocyte elevated gene-1 ( AEG-1), a downstream target of Ras and c-Myc, has been implicated in the development of multiple tumours, but its role in melanoma remains unclear. In the present study, the role of AEG-1 in melanoma was explored through AEG-1 silencing. Our results showed that silencing AEG-1 inhibited the proliferation of melanoma cells, induced cell cycle arrest, and reduced levels of cyclin A, cyclin B, cyclin D1, cyclin E, and cyclin-dependent kinase 2. AEG-1silencing also induced apoptosis in melanoma cells and altered the levels of cleaved caspase-3, B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein. Moreover, silencing AEG-1 suppressed the migration and invasion of melanoma cells, reduced the expressions and activities of matrix metallopeptidase (MMP)-2 and MMP-9, and inhibited the activation of the Wnt/β-catenin signalling pathway in melanoma cells. Furthermore, in vivo experiments revealed that AEG-1 silencing inhibited the growth of melanoma xenografts in nude mice. In summary, our study demonstrates an oncogenic role of AEG-1 in melanoma and suggests that AEG-1 may serve as a potential therapeutic target in the treatment of melanoma.
- Subjects
APOPTOSIS; MELANOMA; CELL proliferation; CATENINS; PROSTATE cancer
- Publication
Clinical & Experimental Pharmacology & Physiology, 2017, Vol 44, Issue 7, p815
- ISSN
0305-1870
- Publication type
Article
- DOI
10.1111/1440-1681.12767