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- Title
Inhibition of NMDA Receptors Underlies the Neuroprotective Effect of Ginsenoside Rb<sub>3</sub>.
- Authors
Liang-Liang Peng; Hong-Mei Shen; Zheng-Lin Jiang; Xia Li; Guo-Hua Wang; Yun-Feng Zhang; Kai-Fu Ke
- Abstract
In order to investigate the mechanisms underlying the neuroprotective effect of ginsenoside Rb3, rat hippocampal neurons were primarily cultured, and exposed to 1 mM N-methyl-D-aspartate (NMDA), cell viability and lactate dehydrogenase leakage were measured. Ca2+ influx was determined by calcium imaging with a laser confocal microscopy. The influences of ginsenoside Rb3 on these variables were examined. Patch-clamp technique was used to observe the effects of ginsenoside Rb3 on NMDA-evoked current. The results show that treatment of Rb3 raised the neuronal viability, reduced the leakage of lactate dehydrogenase, and inhibited NMDA-elicited Ca2+ influx in a dose-dependent manner. In the presence of Rb3, NMDA-evoked peak current was inhibited, and Ca2+-induced desensitization of NMDA current was facilitated. It is suggested that ginsenoside Rb3 could exert a neuroprotective role on hippocampal neurons, a role which was partly mediated by the facilitation of Ca2+-dependent deactivation of NMDA receptors, and the resultant reduction of intracellular free Ca2+ level.
- Subjects
METHYL aspartate; NEUROPROTECTIVE agents; NEURONS; HIPPOCAMPUS (Brain); ASPARTIC acid
- Publication
American Journal of Chinese Medicine, 2009, Vol 37, Issue 4, p759
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X09007223