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- Title
Studies on Glutamate Synthase from the Leaves of Higher Plants.
- Authors
WALLSGROVE, R. M.; HAREL, E.; LEA, P. J.; MIFLIN, B. J.
- Abstract
Ferredoxin-dependent glutamate synthase has been isolated and purified 170-fold from field bean ( cv. Minor) leaves. The enzyme has a molecular weight of 145 000 and does not appear to contain non-haem iron. The values of the enzyme for glutamine, 2-oxoglutarate, and ferredoxin are 330, 150, and 2 μM respectively. The specificity of the enzyme is extremely high and no substitutes for glutamine have been found. Azaserine is a potent inhibitor of glutamate synthase. Chloroplasts from four species of plants have been shown to carry out a light-dependent glutamate synthesis from glutamine. This reaction is also highly specific for glutamine and purified asparagine cannot substitute for glutamine.
- Publication
Journal of Experimental Botany, 1977, Vol 28, Issue 3, p588
- ISSN
0022-0957
- Publication type
Article