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- Title
Effects of Gibberellic Acid on the Activation, Synthesis, and Release of Acid Phosphatase in Barley Seed.
- Authors
BAILEY, K. M.; PHILLIPS, I. D. J.; PITT, D.
- Abstract
Acid phosphatase activity was present in unimbibed barley seed, but rose during incubation of embryoless half-seeds and isolated aleurone layers, and was further increased by 10 M gibberellic acid (GA). Release of total acid phosphatase activity from half-seeds and aleurone layers was markedly enhanced by GA. Inhibitor studies with cycloheximide and actinomycin D suggested that synthesis of acid phosphatase occurred following imbibition. Gel nitration, electrophoresis, and [C]leucine incorporation studies revealed that a single molecular form of acid phosphatase was present in dry seed, whereas on incubation two further forms arose. A proportion of the three molecular forms of the enzyme was synthesized . Gibberellic acid stimulated activation, but not synthesis, of all three molecular forms of acid phosphatase. Although a small amount of one of the molecular forms was secreted in the absence of GA, the presence of gibberellin greatly increased secretion of the same form of acid phosphatase.
- Publication
Journal of Experimental Botany, 1976, Vol 27, Issue 2, p324
- ISSN
0022-0957
- Publication type
Article