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- Title
Modern Methods Of Domestic Animals' Karyotyping Metode Moderne De Realizare A Cariotipului La Animale Domestice.
- Authors
Pusta, Dana; Militaru, Mariela; Szalai, Melinda; Sobolu, Rodica; Paşca, I.
- Abstract
The purpose of the study is to realize the karyotype in different domestic animals using new and original techniques of staining, reading and realizing of the karyogram. To fulfil the proposed goal we used the classical visualization techniques of the chromosomes in lymphocytes sampled form the peripheral blood. We analyzed both metaphases in Giemsa staining and also the chromosomes in G banding. In the study there were used modern methods of chromosomes karyotyping, consisting of a light microscope and a camera which enables to capture the metaphase and to process the chromosomes. The microscope was a motorized Zeiss Axioscop 2 microscope having a capture camera connected with a computer with a software special for the chromosomes karyotyping, called MetaSystem Ikaros. The software is normally used to karyotype the human chromosomes, but it was adapted by us to karyotype the chromosomes in different domestic animals. The size, shape and number of the chromosomes are different and characteristic to each species, so this feature is very important for the studying of the chromosomes. Compared to the classical karyotyping methods, this software is very facile and for this `reason we recommend it to be used to realize the karyotype in animals with increase biological value, especially to those intensively used for reproduction and selection, in order to observe some chromosomal abnormalities and to exclude these animals from reproduction.
- Subjects
KARYOTYPES; CHROMOSOME abnormalities; CELL division; DOMESTIC animal genetics; ANIMAL genome mapping; ANIMAL reproduction
- Publication
Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2009, Vol 66, Issue 1, p472
- ISSN
1843-5270
- Publication type
Article