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- Title
A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops.
- Authors
Moura-Melo, Suely; Miranda-Castro, Rebeca; de-los-Santos-Álvarez, Noemí; Miranda-Ordieres, Arturo J.; Ribeiro dos Santos Junior, José; da Silva Fonseca, Rosana A.; Lobo-Castañón, María Jesús
- Abstract
The design of screening methods for the detection of genetically modified organisms (GMOs) in food would improve the efficiency in their control. We report here a PCR amplification method combined with a sequence-specific electrochemical genosensor for the quantification of a DNA sequence characteristic of the 35S promoter derived from the cauliflower mosaic virus (CaMV). Specifically, we employ a genosensor constructed by chemisorption of a thiolated capture probe and p-aminothiophenol gold surfaces to entrap on the sensing layer the unpurified PCR amplicons, together with a signaling probe labeled with fluorescein. The proposed test allows for the determination of a transgene copy number in both hemizygous (maize MON810 trait) and homozygous (soybean GTS40-3-2) transformed plants, and exhibits a limit of quantification of at least 0.25% for both kinds of GMO lines.
- Subjects
TRANSGENIC organisms; GENETICALLY modified foods; NUCLEOTIDE sequence; CHEMISORPTION; MOSAIC viruses
- Publication
Sensors (14248220), 2017, Vol 17, Issue 4, p881
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s17040881