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- Title
A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma.
- Authors
Yixing Guo; Xiaofeng Yang; Hakuna, Lovemore; Barve, Aabha; Escobedo, Jorge O.; Lowry, Mark; Strongin, Robert M.
- Abstract
A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.
- Subjects
GLUTATHIONE; SULFUR amino acids; HOMOCYSTEINE; FLUORESCENCE spectroscopy; MOLECULAR probes
- Publication
Sensors (14248220), 2012, Vol 12, Issue 5, p5940
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s120505940