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- Title
Recombination-induced CAG trinucleotide repeat expansions in yeast involve the MRE11-RAD50-XRS2 complex.
- Authors
Richard, Guy-Frank; Goellner, Geoffrey M.; McMurray, Cynthia T.; Haber, James E.
- Abstract
Recombination induced by double-strand breaks (DSBs) in yeast leads to a higher proportion of expansions to contractions than does replication-associated tract length changes. Expansions are apparently dependent on the property of the repeat array to form hairpins, since DSB repair of a CAA87 repeat induces only contractions of the repeat sequence. DSB-repair efficiency is reduced by 40% when DNA synthesis must traverse a CAG98 array, as compared with a CAA87 array. These data indicate that repair-associated DNA synthesis is inhibited by secondary structures formed by CAG98 and that these structures promote repeat expansions during DSB repair. Over-expression of Mre11p or Rad50p suppresses the inhibition of DSB repair by CAG98 and significantly increases the average size of expansions found at the recipient locus. Both effects are dependent on the integrity of the Mre11pRad50pXrs2p complex. The Mre11 complex thus appears to be directly involved in removing CAG or CTG hairpins that arise frequently during DNA synthesis accompanying gene conversion of these trinucleotide repeats.
- Subjects
DNA synthesis; DNA replication; DNA repair; BIOCHEMICAL genetics; MOLECULAR genetics; BIOCHEMISTRY
- Publication
EMBO Journal, 2000, Vol 19, Issue 10, p2381
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/19.10.2381