We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Hypouricemia Linked to an Overproduction of Nitric Oxide Is an Early Marker of Oxidative Stress in Female Subjects with Type 1 Diabetes.
- Authors
Pitocco, Dario; Zaccardi, Francesco; Romitelli, Federica; Di Stasio, Enrico; Manto, Andrea; Musella, Tittania; Zuppi, Cecilia; Santini, Stefano A.; Ghirlanda, Giovanni
- Abstract
Introduction: Diabetes reduces the gender protection for cardiovascular disease in premenopausal women. The production of nitric oxide and the increase of oxidative stress is linked to the pathogenesis of diabetic complications. Furthermore nitric oxide, inhibiting the activity of xanthine oxidase, reduces the production of an anti-oxidant as uric acid. Aim of this study was to verify if in an early phase of the disease there is an alteration of oxidative status and if it is linked to the interaction between nitric oxide and uric acid production. Methods: 38 uncomplicated, no smoker female patients with type 1 diabetes (DM) with a duration disease less than 7 years and 25 women comparable for age as control group (C) were enrolled in the study. Total plasma antioxidant capacity (TRAP), Nitric Oxide (NO), Hypoxanthine, Xanthine, Adenosine, Ascorbic Acid, GSSG, GSH, Malonildialdeide, plasmatic and urinary uric acid (UAp and UAu),C-peptide, Glomerular Filtration Rate (GFR) by [sup 51]Cr EDTA, HbA1c, HDL, LDL and triglycerides were measured. Results: Compared with C, DM showed higher NO(42.9±17.9 vs 25.1 ± 12.6, p<0,0001), Xanthine (3.4±0.8 vs 2.6±0.5, p<0,0001), Hypoxanthine (4.4±0.9 vs 3.1±4-0.7, p<0,0001), Adenosine (0.94±0.16 vs 0.77±0.16, p<0.0001), GSSG (4.4 ±0.8 vs 2.8 ± 0.6, p<0.0001),GSH (36.4±6.8 vs 30.1±2.5, p<0.0001),Malonildialdeide (0.018±0.06 vs 0.002±0.002, p<0.0001),HDL (59±0 vs 38 ± 5, p<0.0001)and lower TRAP (581 ± 96.8 vs 941.3 ±80.4, p<0,0001), UAp (2.8±0.6 vs 4.5±1.1 p<0,0001), UAu (9±3 vs 25±6, p<0,0001). No differences were observed about GFR and Ascorbic Acid. Conclusions: Hyperglycaemia could promote, through the activation of NF-κβ, the expression of endothelial iNOS and the generation of NO. NO and not hyperfiltration might reduce AUp and consequently TRAP through the inibition of Xanthine Oxidase as confirmed by higher values of precursor compounds of uric acid synthesis and lower AUu. This could be one of the mechanisms that could explain the early involvement of oxidative status in diabetes.
- Subjects
URIC acid; NITRIC oxide; OXIDATIVE stress; PEOPLE with diabetes; DISEASES in women; DIABETES complications
- Publication
Diabetes, 2007, Vol 56, pA184
- ISSN
0012-1797
- Publication type
Article