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- Title
Proteomics-Based Evidence for a Pro-Oncogenic Role of ESRP1 in Human Colorectal Cancer Cells.
- Authors
Ala, Ugo; Manco, Marta; Mandili, Giorgia; Tolosano, Emanuela; Novelli, Francesco; Provero, Paolo; Altruda, Fiorella; Fagoonee, Sharmila
- Abstract
The RNA-binding protein, Epithelial Splicing Regulatory Protein 1 (ESRP1) can promote or suppress tumorigenesis depending on the cell type and disease context. In colorectal cancer, we have previously shown that aberrantly high ESRP1 expression can drive tumor progression. In order to unveil the mechanisms by which ESRP1 can modulate cancer traits, we searched for proteins affected by modulation of Esrp1 in two human colorectal cancer cell lines, HCA24 and COLO320DM, by proteomics analysis. Proteins hosted by endogenous ESRP1 ribonucleoprotein complex in HCA24 cells were also analyzed following RNA-immunoprecipitation. Proteomics data were complemented with bioinformatics approach to exploit publicly available data on protein-protein interaction (PPI). Gene Ontology was analysed to identify a common molecular signature possibly explaining the pro-tumorigenic role of ESRP1. Interestingly, proteins identified herein support a role for ESRP1 in response to external stimulus, regulation of cell cycle and hypoxia. Our data provide further insights into factors affected by and entwined with ESRP1 in colorectal cancer.
- Subjects
COLORECTAL cancer; IRINOTECAN; CANCER cells; RNA-binding proteins; PROTEOMICS; GENE ontology; PROTEIN-protein interactions; CELL cycle regulation
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 2, p1
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21020575