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- Title
Effects of Dietary Bisphenol A on the Reproductive Function of Gilthead Sea Bream (Sparus aurata) Testes.
- Authors
Forner-Piquer, Isabel; Fakriadis, Ioannis; Mylonas, Constantinos C; Piscitelli, Fabiana; Di Marzo, Vincenzo; Maradonna, Francesca; Calduch-Giner, Josep; Pérez-Sánchez, Jaume; Carnevali, Oliana
- Abstract
Bisphenol A (BPA), a known endocrine disrupting chemical (EDC), was administered by diet to gilthead sea bream (Sparus aurata) in order to study its effects on the endocannabinoid system (ECS) and gonadal steroidogenesis. 2-year-old male gilthead sea bream were fed with two different concentrations of BPA (LOW at 4 and HIGH at 4000 µg/kg body weight for 21 days during the reproductive season. Exposure to 4000 µg BPA/kg bw/day (BPA HIGH) reduced sperm motility and altered the straight-line velocity (VSL) and linearity (LIN). Effects on steroidogenesis were evident, with testosterone (T) being up-regulated by both treatments and 11-ketotestosterone (11-KT) down-regulated by BPA HIGH. Plasma levels of 17β-estradiol (E2) were not affected. The Gonadosomatic Index (GSI) increased in the BPA HIGH group. Interestingly, the levels of endocannabinoids and endocannabinoid-like compounds were significantly reduced after both treatments. Unpredictably, a few changes were noticed in the expression of genes coding for ECS enzymes, while the receptors were up-regulated depending on the BPA dose. Reproductive markers in testis (leptin receptor (lepr), estrogen receptors (era, erb), progesterone receptors (pr) and the gonadotropin releasing hormone receptor (gnrhr)) were up-regulated. BPA induced the up-regulation of the hepatic genes involved in oogenesis (vitellogenin (vtg) and zona pellucida 1 (zp1)).
- Subjects
SPARUS aurata; BISPHENOL A; GONADOTROPIN releasing hormone; ENDOCRINE disruptors; LEPTIN receptors; PROGESTERONE; SEX hormones
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 20, p5003
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20205003