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- Title
Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant (Camellia sinensis) Is Related to the Regulation of Ca<sup>2+</sup>, CaM and Depolarization of Plasma Membrane Potential.
- Authors
Xian-Chen Zhang; Hong-Jian Gao; Tian-Yuan Yang; Hong-Hong Wu; Yu-Mei Wang; Zheng-Zhu Zhang; Xiao-Chun Wan
- Abstract
Tea plant is known to be a hyper-accumulator of fluoride (F). Over-intake of F has been shown to have adverse effects on human health, e.g., dental fluorosis. Thus, understanding the mechanisms fluoride accumulation and developing potential approaches to decrease F uptake in tea plants might be beneficial for human health. In the present study, we found that pretreatment with the anion channel inhibitor NPPB reduced F accumulation in tea plants. Simultaneously, we observed that NPPB triggered Ca2+ efflux from mature zone of tea root and significantly increased relative CaM in tea roots. Besides, pretreatment with the Ca2+ chelator (EGTA) and CaM antagonists (CPZ and TFP) suppressed NPPB-elevated cytosolic Ca2+ fluorescence intensity and CaM concentration in tea roots, respectively. Interestingly, NPPB-inhibited F accumulation was found to be significantly alleviated in tea plants pretreated with either Ca2+ chelator (EGTA) or CaM antagonists (CPZ and TFP). In addition, NPPB significantly depolarized membrane potential transiently and we argue that the net Ca2+ and H+ efflux across the plasma membrane contributed to the restoration of membrane potential. Overall, our results suggest that regulation of Ca2+-CaM and plasma membrane potential depolarization are involved in NPPB-inhibited F accumulation in tea plants.
- Subjects
TEA; FLUORIDES; CELL membranes; MEMBRANE potential; FLUORESCENCE; PLANTS
- Publication
International Journal of Molecular Sciences, 2016, Vol 17, Issue 1, p57
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms17010057