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- Title
3,3'-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence.
- Authors
Jung Kyu Choi; D'Urso, Alessandro; Trauernicht, Mitch; Shabbir-Hussain, Murtaza; Holmes, Andrea E.; Balaz, Milan
- Abstract
Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3'-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatures for all explored DNA sequences upon Cy7 binding. Cy7 was able to spectroscopically discriminate between the right handed B-DNA of poly(dG-dC)2 and its left handed Z-DNA counterpart induced by spermine or Co(III)hexamine via nearly opposite induced circular dichroic signal.
- Subjects
CIRCULAR dichroism; ABSORPTION; NUCLEOTIDE sequence; SPERMINE; BIOGENIC amines; METHENAMINE
- Publication
International Journal of Molecular Sciences, 2011, Vol 12, Issue 11, p8052
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms12118052