We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Prevalence of the HPA-18w to -21w alleles in the Chinese Han population.
- Authors
Liu, Y.; Hong, X.; Xu, X.; Ying, Y.; He, J.; Zhu, F.; Lv, H.; Yan, L.
- Abstract
Recently, four new platelet alloantigen ( HPA) systems HPA-18w to-21w were identified. However, genotyping for HPA-18w to -21w alleles was rarely reported. Here, we established a polymerase chain reaction sequence-based typing ( PCR- SBT) method and investigated the distribution of HPA-18w to -21w alleles in the Chinese Han population. The specific primers of HPA-18w, -19w, -20w and -21w were designed, and the PCR products were bidirectionally sequenced. 855 randomly selected platelet donors were genotyped for HPA-18w to -21w with the PCR- SBT method. The results showed that all individuals were monomorphic for HPA-18w to HPA-20w with a/a homozygous frequency of 1.0 and absence of HPA-18bw to -20bw alleles. The frequencies of the HPA-21a/21a and HPA-21a/21b genotypes were 0.981(839/855) and 0.019(16/855), respectively. Seven mutations were confirmed on sequenced region separate from HPA polymorphisms, including ITGA2 ( IVS17+48G>A and IVS17+72G>A), ITGA2B ( IVS19-26C>G) and ITGB3 ( IVS4+234C>T, IVS11-19 T>C, IVS11-104T>C and GT repeats from IVS11-131 to IVS11-109). These data will provide useful information for diagnosis, prevention and treatment of alloimmune thrombocytopaenia.
- Subjects
POLYMERASE chain reaction; DISEASE prevalence; ALLELES; CHINESE people; ANTIGENS; THROMBOCYTOPENIA; BLOOD platelets
- Publication
International Journal of Immunogenetics, 2013, Vol 40, Issue 2, p99
- ISSN
1744-3121
- Publication type
Article
- DOI
10.1111/j.1744-313X.2012.01142.x