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- Title
Isolation of a FAD-GPDH gene encoding a mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase from Dunaliella salina.
- Authors
Wanggui Yang; Yi Cao; Xiaofei Sun; Fei Huang; Qinghua He; Dairong Qiao; Linhan Bai
- Abstract
The mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase (FAD-GPDH), recently reported in plants, has been detailed in yeast and animal systems. It oxidizes glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) on the outer surface of mitochondrial inner membrane. A cDNA encoding the Dunaliella salina mitochondrial glycerol-3-phosphate dehydrogenase (DsFAD-GPDH) has been cloned and sequenced. The full length cDNA is 2791 bp, with an open reading frame (ORF) encoding 650 predicted amino acids, which show strong homology to reported FAD-GPDHs and have an apparent mitochondrial targeting sequence in the N-terminal. The sequence has been submitted to the GenBank database under Accession No. DQ916107. Results of Real-Time Quantitative PCR and enzymatic assays show that expression of DsFAD-GPDH is enhanced at first by salt treatment, and repressed by oxygen deficiency and cold stress. (© 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
- Subjects
DEHYDROGENASES; AMINO acids; HOMOLOGY (Biology); PHOTOSYNTHETIC oxygen evolution
- Publication
Journal of Basic Microbiology, 2007, Vol 47, Issue 3, p266
- ISSN
0233-111X
- Publication type
Article
- DOI
10.1002/jobm.200610263