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- Title
Transferrin receptor 1 targeted optical imaging for identifying glioma margin in mouse models.
- Authors
Ni, Xiang-Rong; Zhao, Yi-Ying; Cai, Hai-Ping; Yu, Zhi-Hui; Wang, Jing; Chen, Fu-Rong; Yu, Yan-Jiao; Feng, Guo-Kai; Chen, Zhong-Ping
- Abstract
Objective: Optical molecular imaging technology that indiscriminately detects intracranial glioblastoma (GBM) can help neurosurgeons effectively remove tumor masses. Transferrin receptor 1 (TfR 1) is a diagnostic and therapeutic target in GBM. A TfR 1-targeted peptide, CRTIGPSVC (CRT), was shown to cross the blood brain barrier (BBB) and accumulate at high levels in GBM tissues. In this study, we synthesized a TfR 1-targeted near-infrared fluorescent (NIRF) probe, Cy5-CRT, for identifying the GBM tissue margin in mouse models. Methods: We initially confirmed the overexpression of TfR 1 in GBM and the tumor-specific homing ability of Cy5-CRT in subcutaneous and orthotopic GBM mouse models. We then examined the feasibility of Cy5-CRT for identifying the tumor margin in orthotopic GBM xenografts. Finally, we compared Cy5-CRT with the clinically used fluorescein sodium in identifying tumor margins. Results: Cy5-CRT specifically accumulated in GBM tissues and detected the tumor burden with exceptional contrast in mice with orthotopic GBM, enabling fluorescence-guided GBM resection under NIRF live imaging conditions. Importantly, Cy5-CRT recognized the GBM tissue margin more clearly than fluorescein sodium. Conclusions: The TfR 1-targeted optical probe Cy5-CRT specifically differentiates tumor tissues from the surrounding normal brain with high sensitivity, indicating its potential application for the precise surgical removal of GBM.
- Subjects
TRANSFERRIN receptors; OPTICAL images; NANOTECHNOLOGY; GLIOBLASTOMA multiforme; MICE
- Publication
Journal of Neuro-Oncology, 2020, Vol 148, Issue 2, p245
- ISSN
0167-594X
- Publication type
Article
- DOI
10.1007/s11060-020-03527-3