We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
MicroRNA-196a-5p in Extracellular Vesicles Secreted from Myoblasts Suppresses Osteoclast-like Cell Formation in Mouse Cells.
- Authors
Takafuji, Yoshimasa; Tatsumi, Kohei; Kawao, Naoyuki; Okada, Kiyotaka; Muratani, Masafumi; Kaji, Hiroshi
- Abstract
Muscle/bone interaction has been recently noted. Extracellular vesicles (EVs) play a vital role in physiological and pathophysiological processes by transferring microRNA (miRNA) to distant tissues. We previously reported that EVs secreted from C2C12 myoblasts (Myo-EVs) suppress osteoclast differentiation. In the present study, we identified 4 miRNAs in Myo-EVs that suppressed osteoclast-like cell formation in Raw264.7 cells using small RNA sequencing analysis. Among them, miR-196a-5p expression was higher in C2C12 cells compared to mouse osteoblasts and bone marrow cells. Transfection of miR-196a-5p mimic suppressed the mRNA levels of osteoclast-related genes and mitochondrial energy metabolism induced by receptor activator of nuclear factor-κB ligand in Raw264.7 cells. In contrast, miR-196a-5p mimic enhanced osteoblastic differentiation in ST-2 cells and MC3T3-E1 cells. In conclusion, we demonstrated that miR-196-5p suppresses osteoclast-like cell formation and mitochondrial energy metabolism in mouse cells, suggesting that it might be a crucial factor for muscle/bone interaction via EVs.
- Subjects
OSTEOCLASTOGENESIS; EXTRACELLULAR vesicles; MYOBLASTS; BONE marrow cells; RNA sequencing; NON-coding RNA; CELLS
- Publication
Calcified Tissue International, 2021, Vol 108, Issue 3, p364
- ISSN
0171-967X
- Publication type
journal article
- DOI
10.1007/s00223-020-00772-6