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- Title
DNA affinity binding studies using a fluorescent dye displacement technique: the dichotomy of the binding site.
- Authors
Spillane, Caitriona B.; Smith, Jayden A.; Morgan, Joy L.; Keene, F. Richard
- Abstract
We have observed a number of discrepancies and contradictions in the use of a fluorescent intercalator displacement assay in surveying the binding affinities of dinuclear polypyridyl ruthenium(II) complexes with DNA. By a modification of the assay using the fluorescent minor-groove binder 4′,6-diamidino-2-phenylindole, rather than intercalating dyes (ethidium bromide or thiazole orange), results were obtained for all complexes studied which were consistent with relative affinities and stereoselectivities observed with other techniques, including NMR, affinity chromatography and equilibrium dialysis. It is believed that the difference in binding mode between the minor groove-binding Ru(II) complexes and the intercalating fluorescent dyes they are displacing may contribute to these discrepancies.
- Subjects
DNA; RUTHENIUM; BROMIDES; NUCLEAR magnetic resonance; DIALYSIS (Chemistry); CHROMATOGRAPHIC analysis
- Publication
Journal of Biological Inorganic Chemistry (JBIC), 2007, Vol 12, Issue 6, p819
- ISSN
0949-8257
- Publication type
Article
- DOI
10.1007/s00775-007-0235-9