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- Title
Enhanced production of exopolysaccharides using industrial grade starch as sole carbon source.
- Authors
He, Xun; He, Feng; Hang, Jiao; Li, Hui; Chen, Yali; Wei, Ping; Chen, Kequan; Li, Yan; OuYang, Pingkai
- Abstract
Industrial grade soluble corn starch was used directly and effectively as the fermentation substrate for microbial exopolysaccharides production. Bacillus subtilis mutant strain NJ308 grew with untreated starch raw material as the sole carbon source. The real-time PCR results demonstrated that up-regulated genes encoding N-acetylglucosaminyltransferase, mannosyltransferase, and N-acetylglucosamine-1-phosphate uridyltransferase were the key elements of B. subtilis mutant strain NJ308 for exopolysaccharides production from industrial grade starch. Subsequently, the culture conditions for B. subtilis NJ308 were optimized using Plackett-Burman design and central composite design methods, and the related key genes in the synthesis pathway of exopolysaccharides from the starch raw material were analyzed by real-time PCR. The maximum exopolysaccharides titration (3.41 g/L) was obtained when the initial starch concentration was 45 g/L. This corresponds to volumetric productivity values of 71.04 mg/L h.
- Subjects
STARCH; BACILLUS subtilis; CARBON; FERMENTATION; POLYMERASE chain reaction
- Publication
Bioprocess & Biosystems Engineering, 2018, Vol 41, Issue 6, p811
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-018-1915-2