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- Title
Molecular Characterization of Cryptic Species of Bemisia tabaci Associated with Cucumber in Eastern Dry Zone of Karnataka.
- Authors
SINDHURA, KOPPARTHI AMRUTHA VALLI; REDDY, K. M. SRINIVAS; POOJA, P. S.; BHARATH, M.; KHATIB, SHADAB M.; SUMITHRAMMA, N.; REDDY, G. KESHAVA; REDDY, C. N. LAKSHMINARAYANA
- Abstract
The whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is posing major havoc on vegetable production jeopardizing Indian agriculture. During, April 2023, whitefly, B. tabaci sample was collected from the cucumber fields of Muthagadahalli village in the Bengaluru North taluk, Bengaluru district of Karnataka. The total genomic DNA was extracted from a single adult whitefly using the Chelex 100 method. The identification of whitefly cryptic species was performed by amplifying the mitochondrial cytochrome oxidase subunit one (mtCOI) gene using gene-specific primers of B. tabaci. PCR results revealed expected amplificon size of 800 bp. Subsequently, the PCR product was subjected to Sanger sequencing. The nucleotide (nt) sequence of the whitefly sample collected from Muthagadahalli exhibited 98-100 per cent identity with sequences belonging to Asia I whitefly cryptic species known to infect different crops worldwide. The sequence demarcation graph and phylogenetic analysis provided substantial evidence for the present findings.
- Subjects
KARNATAKA (India); BENGALURU (India); SWEETPOTATO whitefly; CYTOCHROME oxidase; SPECIES; ALEYRODIDAE; HEMIPTERA
- Publication
Mysore Journal of Agricultural Sciences, 2023, Vol 57, Issue 4, p175
- ISSN
0047-8539
- Publication type
Article