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- Title
Establishment of Recombinase Polymerase Amplification for Rapid Detection of Spring Viremia of Carp Virus (SVCV).
- Authors
Liang Junni; Yin Weili; Shang Di; Liu Ning; Duan Xiaohui; Lin Sen
- Abstract
[Objective] The paper was to develop a rapid method for the detection of spring Viremia of carp virus (SVCV). [Method] The specific primers were designed by targeting the G gene of SVCV. The recombinase polymerase amplification (RPA) assay for detecting SVCV was established by optimizing the reaction conditions. The optimal amplification temperature of RPA assay was 30"C, and the test could be finished within 20 min. [Result] The method was specific with no cross-reaction with other common fish infectious viruses. Sensitivity test showed that the lowest detection limit of the method was 89.2 copies/µ.L, higher than that of traditional RT-PCR. Moreover, a total of 80 clinical samples were detected by RPA and RT-PCR, respectively. The weak positive samples tested by RT-PCR could be detectable with RPA, indicating that RPA assay could be used in clinical detection. [Conclusion] The method established is rapid, simple, specific and sensitive for testing SVCV, and it will be widely used in grassroots laboratory and on-site inspection.
- Subjects
CARP; CROSS reactions (Immunology); VIREMIA; SPRING; DETECTION limit
- Publication
Animal Husbandry & Feed Science, 2020, Vol 12, Issue 2, p29
- ISSN
1943-9911
- Publication type
Article
- DOI
10.19578/j.cnki.ahfs.2020.02.006