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- Title
Reproducibility of Non-Invasive A Adenosine Receptor Quantification in the Rat Brain Using [F]CPFPX and Positron Emission Tomography.
- Authors
Kroll, Tina; Elmenhorst, David; Weisshaupt, Angela; Beer, Simone; Bauer, Andreas
- Abstract
Purpose: The AAR antagonist 8-cyclopentyl-3-(3-fluoropropyl)-1-propylxanthine ([F]CPFPX) has recently been shown to be a suitable radiotracer for quantitative in vivo imaging of the A adenosine receptor (AAR) in rats. The present study evaluates the reproducibility of non-invasive longitudinal AAR studies with [F]CPFPX and a dedicated small animal positron emission tomography (PET) scanner. Procedures: Twelve male Sprague Dawley rats underwent four repeated dynamic PET scans with a bolus injection of [F]CPFPX. AAR availability was determined by different non-invasive approaches including simplified and multilinear reference tissue (olfactory bulb)-based models and graphical methods. The outcome parameter binding potential ( BP) was evaluated in terms of variability and reproducibility. Results: Repeated estimations of [F]CPFPX BP gave reliable results with acceptable variability (mean 12 %) and reproducibility (intraclass correlation coefficients raging from 0.57 to 0.68) in cortical and subcortical regions of the rat brain. With regard to kinetic models, test-retest stability of the simplified reference-tissue model (SRTM) was superior to multilinear and graphical approaches. Conclusions: Non-invasive quantification of AAR density in the rat brain is reproducible and reliable with [F]CPFPX PET and allows longitudinal designs of in vivo imaging studies in rodents.
- Subjects
POSITRON emission tomography; LABORATORY rats; OLFACTORY bulb; BRAIN imaging; ADENOSINES; RADIOACTIVE tracers
- Publication
Molecular Imaging & Biology, 2014, Vol 16, Issue 5, p699
- ISSN
1536-1632
- Publication type
Article
- DOI
10.1007/s11307-014-0729-0