We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
UBE2T mediates the stemness properties of breast cancer cells through the mTOR signaling pathway.
- Authors
JIAWEI YIN; YONGSHENG WANG; GUANGWEI WEI; MINGXIN WEN
- Abstract
Objectives: This study aimed to reveal the role and possible mechanism of the ubiquitin-conjugating enzyme 2T (UBE2T) in the biological activities of breast cancer stem cells (BCSCs). Methods: The specific protein and gene expression were quantified by Western blotting and quantitative real-time polymerase chain reaction, the proportion of BCSCs was examined by flow cytometry, and the self-renewal and proliferation of BCSCs were verified by serial sphere formation and soft agar. Results: Increasing expression of UBE2T was drastically found in breast cancer than that in adjacent tissues. Furthermore, UBE2T overexpression significantly increased the proportion of BCSCs in breast cancer cells and promoted their self-renewal and proliferation. Silent UBE2T exhibited the opposite functions. UBE2T increased the levels of the mammalian target of rapamycin and the phosphorylated mammalian target of rapamycin. Mammalian target of rapamycin (mTOR) inhibitor rapamycin inhibited the function of UBE2T in BCSCs. Conclusion: UBE2T plays a role in BCSCs through mTOR pathway and may suggest a novel therapeutic strategy for breast cancer.
- Subjects
UBIQUITIN-conjugating enzymes; BREAST cancer; CANCER cells; CELLULAR signal transduction; GENE expression; RAPAMYCIN
- Publication
Biocell, 2024, Vol 48, Issue 6, p959
- ISSN
0327-9545
- Publication type
Article
- DOI
10.32604/biocell.2024.049349