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- Title
microRNA 490-3P enhances the drug-resistance of human ovarian cancer cells.
- Authors
Shuo Chen; Xi Chen; Yin-Ling Xiu; Kai-Xuan Sun; Zhi-Hong Zong; Yang Zhao
- Abstract
Background MicroRNAs (miRNAs) are non-coding, single-stranded small RNAs that regulate gene expression negatively, which is involved in fundamental cellular processes. In this study, we investigated the role of mi-490-3P in the development of drug resistance in ovarian cancer cells. Methods The human ovarian carcinoma cell line A2780 and A2780/Taxol were exposed to paclitaxel in the presence or absence of microRNA 490-3P transfection, after which cell viability were performed by CCK-8 assay. Reverse transcription polymerase chain reaction (RT-PCR) and western blotting were used to assess the mRNA and protein expression levels of GST-π, MDR1 or P-gp. Results Our results showed higher mi-490-3P mRNA expression level in A2780/Taxol cells than in A2780 cells (p < 0.05). Following mi-490-3P transfection, both A2780 and A2780/Taxol cells showed decreased sensitivity to paclitaxel. The mRNA expression levels of MDR1, GST-π (p < 0.05) and protein expression levels of P-gp, GST-π were down-regulated after mi-490-3P transfection in comparison to mock and negative control cancer cells. Conclusion Our results demonstrate for the first time that microRNA 490-3P may be involved in the development of drug resistance in ovarian cancer.
- Subjects
MICRORNA; DRUG resistance; OVARIAN cancer patients; OVARIAN cancer treatment; OVARIAN cancer diagnosis
- Publication
Journal of Ovarian Research, 2014, Vol 7, Issue 1, p1
- ISSN
1757-2215
- Publication type
Article
- DOI
10.1186/s13048-014-0084-4